Evaluation of the toxicity effects of silk fibroin on human lymphocytes and monocytes.
Parvaneh NaserzadehSeyed Alireza MortazaviKhadijeh AshtariAhmad SalimiMehdi FarokhiJalal PourahmadPublished in: Journal of biochemical and molecular toxicology (2018)
Silk fibroin nanoparticles (SFNPs) as a natural polymer have been utilized in biomedical applications such as suture, tissue engineering-based scaffolds, and drug delivery carriers. Since there is little data regarding the toxicity effects on different cells and tissues, we aimed to determine the toxicity mechanisms of SFNPs on human lymphocytes and monocytes based on reliable methods. Our results showed that SFNPs (0.5, 1, and 2 mg/mL) induced oxidative stress via increasing reactive oxygen species production, mitochondrial membrane potential (∆Ψ) collapse, which was correlated to cytochrome c release and Adenosine diphosphate (ADP)/Adenosine tri phosphate (ATP) ratio increase as well as lysosomal as another toxicity mechanism, which led to cytosolic release of lysosomal digestive proteases, phosphor lipases, and apoptosis signaling. Taken together, these data suggested that SFNPs toxicity was associated with mutual mitochondrial/lysosomal cross-talk and oxidative stress on human lymphocytes and monocytes with activated apoptosis signaling.
Keyphrases
- oxidative stress
- tissue engineering
- induced apoptosis
- endothelial cells
- peripheral blood
- cell cycle arrest
- drug delivery
- diabetic rats
- ischemia reperfusion injury
- dna damage
- dendritic cells
- reactive oxygen species
- induced pluripotent stem cells
- endoplasmic reticulum stress
- gene expression
- pluripotent stem cells
- electronic health record
- immune response
- hydrogen peroxide
- nitric oxide
- climate change
- signaling pathway
- machine learning
- oxide nanoparticles
- quantum dots
- risk assessment
- light emitting