Cell Type-Specific Circuit Mapping Reveals the Presynaptic Connectivity of Developing Cortical Circuits.
Laura A CocasGloria FernandezMariya BarchJason DollIvan Zamora DiazSamuel J PleasurePublished in: The Journal of neuroscience : the official journal of the Society for Neuroscience (2016)
Mapping cortical connectivity in the developing mammalian brain has been an intractable problem, in part because it has not been possible to analyze connectivity with cell subtype precision. Our study systematically targets the presynaptic connections of discrete neuronal subtypes in both the mature and developing cerebral cortex. We analyzed the connections that Cajal-Retzius cells make and receive, and found that these cells receive inputs from deeper-layer excitatory neurons and inhibitory interneurons in the first postnatal week. We assessed the inputs onto inhibitory interneurons and excitatory projection neurons, the major two types of neurons in the cortex, and found that excitatory inputs are refined by the fourth week of development, whereas local inhibitory inputs increase during this postnatal period.
Keyphrases
- resting state
- functional connectivity
- induced apoptosis
- white matter
- spinal cord
- cell cycle arrest
- preterm infants
- high resolution
- cerebral ischemia
- endoplasmic reticulum stress
- randomized controlled trial
- single cell
- subarachnoid hemorrhage
- high density
- spinal cord injury
- magnetic resonance
- clinical trial
- oxidative stress
- mass spectrometry
- cell death
- brain injury
- study protocol
- image quality
- cone beam