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Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.

Mateus Maldonado CarrieroFlavio Henrique-SilvaCaroline Munhoz MeiraIgor Mateus Queiroz GatoAlexandre Rodrigues CaetanoFrancisco Pereira LoboAnderson Luis AlvesEduardo Sousa VarelaAntonio Augusto Mendes Maia
Published in: Genetics and molecular biology (2020)
In the present study, the complete characterization of cDNA and genomic sequences of IL-1β and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1β cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1β, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1β and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.
Keyphrases
  • amino acid
  • gene expression
  • genome wide
  • dna methylation
  • poor prognosis
  • binding protein
  • transcription factor
  • long non coding rna
  • endoplasmic reticulum stress
  • wound healing
  • anti inflammatory
  • fluorescent probe