A novel MAP kinase-interacting protein MoSmi1 regulates development and pathogenicity in Magnaporthe oryzae.
Yu WangXinyue CuiJunlian XiaoXiaoru KangJinmei HuZhicheng HuangNa LiChuyu YangYuemin PanShulin ZhangPublished in: Molecular plant pathology (2024)
The cell wall is the first barrier against external adversity and plays roles in maintaining normal physiological functions of fungi. Previously, we reported a nucleosome assembly protein, MoNap1, in Magnaporthe oryzae that plays a role in cell wall integrity (CWI), stress response, and pathogenicity. Moreover, MoNap1 negatively regulates the expression of MoSMI1 encoded by MGG_03970. Here, we demonstrated that deletion of MoSMI1 resulted in a significant defect in appressorium function, CWI, cell morphology, and pathogenicity. Further investigation revealed that MoSmi1 interacted with MoOsm1 and MoMps1 and affected the phosphorylation levels of MoOsm1, MoMps1, and MoPmk1, suggesting that MoSmi1 regulates biological functions by mediating mitogen-activated protein kinase (MAPK) signalling pathway in M. oryzae. In addition, transcriptome data revealed that MoSmi1 regulates many infection-related processes in M. oryzae, such as membrane-related pathway and oxidation reduction process. In conclusion, our study demonstrated that MoSmi1 regulates CWI by mediating the MAPK pathway to affect development and pathogenicity of M. oryzae.
Keyphrases
- cell wall
- single cell
- protein kinase
- biofilm formation
- signaling pathway
- oxidative stress
- poor prognosis
- rna seq
- binding protein
- gene expression
- nitric oxide
- pi k akt
- stem cells
- escherichia coli
- tyrosine kinase
- hydrogen peroxide
- machine learning
- amino acid
- cystic fibrosis
- dna methylation
- long non coding rna
- candida albicans
- bone marrow
- small molecule
- drug induced