Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis.
Tomasz SzaflikHanna RomanowiczKrzysztof SzyłłoRadosław KołacińskiMagdalena M MichalskaDariusz SamulakBeata SmolarzPublished in: International journal of molecular sciences (2022)
Endometriosis is a disease of complex etiology. Hormonal, immunological, and environmental factors are involved in its formation. In recent years, special attention has been paid to genetic mechanisms that can have a significant impact on the increased incidence of endometriosis. The study aimed to analyze the expression of four long non-coding RNA (lncRNA) genes, UCA1, MALAT1, TC0101441, and H19, in the context of the risk of developing endometriosis. The material for genetic testing for the expression of lncRNA genes were tissue slices embedded in paraffin blocks from patients with endometriosis ( n = 100) and the control group ( n = 100). Gene expression was determined by the RT-PCR technique. The expression of the H19 gene in endometriosis patients was statistically significantly lower than in the control group. A statistically significant association was found between H19 gene expression in relation to The Revised American Society for Reproductive Medicine classification of endometriosis (rASRM) in the group of patients with endometriosis. Research suggests that H19 expression plays an important role in the pathogenesis of endometriosis.
Keyphrases
- long non coding rna
- poor prognosis
- gene expression
- genome wide
- end stage renal disease
- chronic kidney disease
- machine learning
- binding protein
- type diabetes
- newly diagnosed
- ejection fraction
- risk factors
- metabolic syndrome
- copy number
- adipose tissue
- deep learning
- transcription factor
- prognostic factors
- patient reported outcomes