Placental growth factor and Fms related tyrosine kinase-1 are hypomethylated in preeclampsia placentae.
Kinjal M DaveLovejeet KaurKaruna N RandhirSavita S MehendaleDeepali P SundraniGiriraj Ratan ChandakSadhana R JoshiPublished in: Epigenomics (2021)
Aim: This study aims to examine the DNA methylation (DNAm) and expression patterns of genes associated with placental angiogenesis in preeclampsia. Materials & methods: DNAm and expression were examined in normotensive (n = 100) and preeclampsia (n = 100) women using pyrosequencing and quantitative real-time PCR respectively. Results: Hypomethylation at several CpGs was observed in PlGF and FLT-1 in women with preeclampsia compared to normotensive controls. PlGF expression was lower in women with preeclampsia while FLT-1 expression was comparable. DNAm at various CpGs was negatively correlated with expression in both the genes and were associated with maternal blood pressure and birth outcomes. Conclusion: DNAm and expression of angiogenic factors in placentae are differentially regulated in preeclampsia and influence birth outcomes.
Keyphrases
- tyrosine kinase
- poor prognosis
- pregnancy outcomes
- early onset
- blood pressure
- dna methylation
- growth factor
- binding protein
- long non coding rna
- acute myeloid leukemia
- epidermal growth factor receptor
- type diabetes
- genome wide
- pregnant women
- transcription factor
- physical activity
- insulin resistance
- endothelial cells
- mass spectrometry
- nk cells