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New parameters for in vitro development of cell lines of the species Astyanax bimaculatus (Linnaeus, 1758) and Geophagus proximus (Castelnau, 1855).

Ivanete de Oliveira FuroLygia S NogueiraRodrigo Petry Corrêa de SousaGlaucia Caroline Silva-OliveiraDiovanna Mirella Dos Santos da SilvaAllan Costa-MalaquiasEdivaldo Herculano Correia de Oliveira
Published in: Journal of fish biology (2024)
Intending to compare in vitro cell growth in different conditions, we established cell cultures using fin biopsies of two freshwater fishes, Astyanax bimaculatus and Geophagus proximus. Three different culture media (Leibovitz-L-15, Dulbecco's Modified Eagle Medium [DMEM], and 199) were employed, with or without the addition of AmnioMax, maintaining a standard temperature of 29°C. Based on the results obtained, we standardized a cell growth protocol in which medium 199 was less efficient for both species. Notably, G. proximus cells exhibited superior proliferation in DMEM and L-15 media, whereas A. bimaculatus cells demonstrated better parameters exclusively in the DMEM medium. Successful subculturing of cells with good proliferation index was observed, accompanied by preserved morphological characteristics. Therefore, the methodology outlined in this study represents an advancement in establishing fish cell cultures.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • signaling pathway
  • single cell
  • endoplasmic reticulum stress
  • randomized controlled trial
  • stem cells
  • cell death
  • pi k akt
  • genetic diversity