Neuroprotection of the hypoxic-ischemic mouse brain by human CD117+CD90+CD105+ amniotic fluid stem cells.
Michelangelo CorcelliKate HawkinsFilipa VlahovaAvina HunjanKate DowdingPaolo De CoppiAnna Louise DavidDonald PeeblesPierre GressensHenrik HagbergMariya HristovaPascale V GuillotPublished in: Scientific reports (2018)
Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43-45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-hAFSCs failed to show such neuro-protective effects. It is possible that SS-hAFSCs exert their neuroprotection via endoglin-dependent inhibition of TGFβ1 signaling in target cells. These findings identify a sub-population of CD117+CD90+CD105+ stem cells as a promising source for the neuro-protection of the developing brain.
Keyphrases
- stem cells
- endothelial cells
- induced apoptosis
- cell therapy
- cell cycle arrest
- healthcare
- pluripotent stem cells
- mesenchymal stem cells
- umbilical cord
- transforming growth factor
- brain injury
- type diabetes
- inflammatory response
- adipose tissue
- resting state
- multiple sclerosis
- functional connectivity
- lps induced
- cell proliferation
- epithelial mesenchymal transition
- tissue engineering