Ganoderma lucidum, a medicinal mushroom with a long history in traditional Chinese medicine, is widely used for chronic diseases. Ganospirones A-G (1-7), seven pairs of undescribed spiro-meroterpenoids, were isolated from the fruiting bodies of G. lucidum. Their structures including absolute configurations were characterized by using NMR spectroscopic data, ECD computational and X-ray diffraction methods. The anti-inflammatory and anti-renal fibrosis activities of the meroterpenoids 1-7 were tested, and the results revealed that (-)-2 and (+)-2 could inhibit iNOS expression in lipopolysaccharide-induced RAW264.7 cells at 20 μM.
Keyphrases
- lipopolysaccharide induced
- high resolution
- inflammatory response
- anti inflammatory
- induced apoptosis
- poor prognosis
- cell cycle arrest
- magnetic resonance
- molecular docking
- endoplasmic reticulum stress
- electron microscopy
- big data
- single cell
- signaling pathway
- oxidative stress
- binding protein
- mass spectrometry
- solid state
- dual energy
- nitric oxide
- computed tomography
- cell proliferation
- data analysis
- deep learning
- liver fibrosis
- molecular dynamics simulations