The inhibitor of apoptosis protein MoBir1 is involved in the suppression of hydrogen peroxide-induced fungal cell death, reactive oxygen species generation, and pathogenicity of rice blast fungus.
Lisha ZhangKaili ZhongRuili LvXiaobo ZhengZhengguang ZhangHaifeng ZhangPublished in: Applied microbiology and biotechnology (2019)
The inhibitor of apoptosis protein (IAP) family has been identified in a variety of organisms. All IAPs contain one to three baculoviral IAP repeat (BIR) domains, which are required for anti-apoptotic activity. Here, we identified a type II BIR domain-containing protein, MoBir1, in the rice blast fungus Magnaporthe oryzae. Expression of the MoBIR1 gene in Saccharomyces cerevisiae suppressed hydrogen peroxide-induced cell death and delayed yeast cell chronological aging. Delayed aging was found to require the carboxyl terminus of MoBir1. M. oryzae transformants overexpressing the MoBIR1 gene demonstrated increased growth rate and biomass, delayed mycelial aging, and enhanced resistance to hydrogen peroxide but reduced reactive oxygen species generation and virulence. Moreover, MoBIR1-overexpressing transformants exhibited anti-apoptotic activity. However, MoBIR1 silencing resulted in no obvious phenotypic changes, compared with the wild-type M. oryzae strain Guy11. Our findings broaden the knowledge on fungal type II BIR domain-containing proteins.
Keyphrases
- hydrogen peroxide
- cell death
- cell cycle arrest
- reactive oxygen species
- saccharomyces cerevisiae
- nitric oxide
- high glucose
- wild type
- binding protein
- diabetic rats
- protein protein
- copy number
- healthcare
- oxidative stress
- escherichia coli
- drug induced
- poor prognosis
- pseudomonas aeruginosa
- wastewater treatment
- genome wide
- single cell
- cell wall
- biofilm formation
- gene expression
- cell therapy
- endoplasmic reticulum stress
- mesenchymal stem cells
- cystic fibrosis
- pi k akt
- long non coding rna
- genome wide identification
- signaling pathway
- dna methylation
- transcription factor