CD8+ T-cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis.
Laura Lorena García-LópezMónica Vargas-MontesJuan Felipe Osorio-MéndezNéstor CardonaAlejandro Hernández De Los RíosCarlos Andrés Toro-AcevedoJuan Camilo Arenas-GarcíaLuz Eliana Mantilla-MurielElizabeth TorresJuan D Valencia-HernándezAlejandro Acosta-DávilaAlejandra de-la-TorreDaniel Celis-GiraldoManuela Mejía OquendoJuan Carlos Sepúlveda-AriasJorge Enrique Gómez-MarínPublished in: Ocular immunology and inflammation (2023)
This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis ( n = 9), chronic asymptomatic toxoplasmosis ( n = 7), and non-infected people ( n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals ( p = .003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.