Induced expression of cathelicidins in trout (Oncorhynchus mykiss) challenged with four different bacterial pathogens.
Michela FurlanUmberto RosaniStefano GambatoPaola IratoAmedeo ManfrinMario MardirossianPaola VenierAlberto PallaviciniMarco ScocchiPublished in: Journal of peptide science : an official publication of the European Peptide Society (2018)
Cathelicidins are an important family of antimicrobial peptide effectors of innate immunity in vertebrates. Two members of this group, CATH-1 and CATH-2, have been identified and characterized in teleosts (ray-finned fish). In this study, we investigated the expression of these genes in different tissues of rainbow trout challenged with 4 different inactivated pathogens. By using qPCR, we detected a strong induction of both cath-1 and cath-2 genes within 24 hours after intraperitoneal inoculation with Lactococcus garvieae, Yersinia ruckeri, Aeromonas salmonicida, or Flavobacterium psychrophilum cells. Up to 700-fold induction of cath-2 was observed in the spleen of animals challenged with Y. ruckeri. Moreover, we found differences in the intensity and timing of gene up-regulation in the analyzed tissues. The overall results highlight the importance of cathelicidins in the immune response mechanisms of salmonids.
Keyphrases
- poor prognosis
- genome wide
- immune response
- genome wide identification
- gene expression
- induced apoptosis
- gram negative
- genome wide analysis
- binding protein
- antimicrobial resistance
- dna methylation
- copy number
- high intensity
- high glucose
- cell cycle arrest
- diabetic rats
- dendritic cells
- signaling pathway
- multidrug resistant
- toll like receptor