Regulation of zinc homeostatic genes by environmental pH in the filamentous fungus Aspergillus fumigatus.
Héctor ToledoClara Inés SánchezLaura MarínJorge AmichJosé Antonio CaleraPublished in: Environmental microbiology (2021)
Aspergillus fumigatus can grow over a broad range of pH values even though zinc availability is greatly conditioned by ambient pH. It has been previously shown that regulation of zinc homeostatic genes in this fungus relies on the transcription factor ZafA. In addition, their expression is further modulated by the transcription factor PacC depending on ambient pH, which allows this fungus to grow in diverse types of niches, including soils and the lungs of immunosuppressed hosts. In this work the regulation by PacC of genes zrfB and zrfC that are expressed, respectively, under acidic and alkaline zinc-limiting conditions have been analysed in detail. Thus, data that extend the current model for PacC function, including the role of the full-length PacC72 protein and the PacC processed forms (PacC53 and PacC27 ) on gene expression has been provided, and a new mechanism for the repression of acid-expressed genes in alkaline media based on interference with the start of transcription has been described. Moreover, it was proposed that the transcription of both acid-expressed and alkaline-expressed genes under zinc-limiting conditions might also rely on a third factor (putatively Pontin/Reptin), which may be required to integrate the action of PacC and ZafA into gene specific transcriptional responses.
Keyphrases
- transcription factor
- genome wide identification
- genome wide
- gene expression
- bioinformatics analysis
- oxide nanoparticles
- dna methylation
- air pollution
- genome wide analysis
- particulate matter
- poor prognosis
- dna binding
- heavy metals
- anaerobic digestion
- binding protein
- long non coding rna
- small molecule
- big data
- electronic health record
- protein protein
- climate change