Interactions of Silver Nanoparticles Formed in Situ on AFM Tips with Supported Lipid Bilayers.
Xitong LiuPublished in: Langmuir : the ACS journal of surfaces and colloids (2018)
A facile approach for functionalizing atomic force microscopy (AFM) tips with nanoparticles (NPs) will provide exciting opportunities in the field of tip-enhanced vibrational spectroscopy and in probing the interactions between NPs and biological systems. In this study, through successive exposure to polydopamine and AgNO3 solutions, the apex of AFM tips was functionalized with silver nanoparticles (AgNPs). The AgNP-modified AFM tips were used to measure the interaction forces between AgNPs and supported lipid bilayers (SLBs) formed on mica, as well as to probe the penetration of SLBs by AgNPs, with an emphasis on the effect of human serum albumin (HSA) proteins. AgNPs experienced predominantly repulsive forces when approaching SLBs. The presence of HSA resulted in an enhancement in the repulsive interactions between AgNPs and SLBs, likely through steric repulsion. Finally, the forces required for AgNPs to penetrate SLBs were higher in the presence of HSA probably due to the increase in the effective size of the nanoscale protuberances on the AFM tip stemming from the formation of protein coronas around the AgNPs.