Modulation of the E-cadherin in human cells infected in vitro with Coxiella burnetii.
Ikram Omar OsmanSoraya MezouarDjamal Brahim-BelhaouariJean-Louis MegeChristian Albert DevauxPublished in: PloS one (2023)
High concentration of soluble E-cadherin (E-cad) was previously found in sera from Q fever patients. Here, BeWo cells which express a high concentration of E-cad were used as an in vitro model to investigate the expression and function of E-cad in response to infection by Coxiella burnetii, the etiological agent of Q fever. Infection of BeWo cells with C. burnetii leads to a decrease in the number of BeWo cells expressing E-cad at their membrane. A shedding of soluble E-cad was associated with the post-infection decrease of membrane-bound E-cad. The modulation of E-cad expression requires bacterial viability and was not found with heat-inactivated C. burnetii. Moreover, the intracytoplasmic cell concentration of β-catenin (β-cat), a ligand of E-cad, was reduced after bacterial infection, suggesting that the bacterium induces modulation of the E-cad/β-cat signaling pathway and CDH1 and CTNNB1 genes transcription. Finally, several genes operating the canonical Wnt-Frizzled/β-cat pathway were overexpressed in cells infected with C. burnetii. This was particularly evident with the highly virulent strain of C. burnetii, Guiana. Our data demonstrate that infection of BeWo cells by live C. burnetii modulates the E-cad/β-cat signaling pathway.
Keyphrases
- coronary artery disease
- induced apoptosis
- signaling pathway
- cell cycle arrest
- endoplasmic reticulum stress
- poor prognosis
- pi k akt
- oxidative stress
- stem cells
- transcription factor
- epithelial mesenchymal transition
- cell death
- newly diagnosed
- mesenchymal stem cells
- single cell
- electronic health record
- big data
- patient reported outcomes
- genome wide identification