Suppressive Effect of Coffee Leaves on Lipid Digestion and Absorption In Vitro.
Veerawat SansriMorakot SroyrayaPochamana PhisalprapaAtchariya YosboonruangAtcharaporn OntawongSurasak SaokaewBey-Hing GohKanittaporn TrisatPremchirakorn PhewchanAnchalee RawangkanNanteetip LimpeanchobAcharaporn DuangjaiPublished in: Foods (Basel, Switzerland) (2024)
Coffee leaf extracts from Arabica coffee contain a high amount of caffeine, whereas extracts from Robusta coffee contain high amounts of chlorogenic acid (CGA) and caffeine. Additionally, leaf extracts from Arabica and Robusta coffee demonstrated the inhibition of pancreatic lipase, decreased micellar cholesterol solubility, and reduced bile acid binding. Furthermore, these extracts resulted in a reduction in cholesterol uptake in Caco-2 cells. Molecular docking experiments supported this discovery, showing CGA and caffeine binding to Niemann-Pick C1-like 1 (NPC1L1), a key protein in cholesterol absorption. The results indicated that CGA and caffeine can competitively bind to NPC1L1 at the cholesterol binding pocket, reducing its cholesterol binding rate. These findings suggest that coffee leaves might help suppress lipid absorption and digestion, highlighting their potential use in preventing and treating hyperlipidemia.
Keyphrases
- low density lipoprotein
- molecular docking
- binding protein
- induced apoptosis
- molecular dynamics simulations
- small molecule
- dna binding
- type diabetes
- fatty acid
- cell proliferation
- high throughput
- oxidative stress
- risk assessment
- insulin resistance
- signaling pathway
- cell death
- human health
- climate change
- protein protein