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An industrially applicable Escherichia coli platform for bioconversion of thebaine to oripavine and codeine to morphine.

Garrick W K SpencerXu LiAilsa JarroldSally L Gras
Published in: Chemical communications (Cambridge, England) (2023)
A whole cell Escherichia coli biotransformation platform converting thebaine to oripavine and codeine to morphine was demonstrated with industrially applicable yields (∼1.2 × 10 -2 g L -1 h -1 or ∼1.2 × 10 -1 g L -1 h -1 ), improving >13 400-fold upon morphine production in yeast. Mutations enhanced enzyme performance and the use of a purified substrate with rich raw poppy extract expanded applicability.
Keyphrases
  • escherichia coli
  • high throughput
  • single cell
  • biofilm formation
  • oxidative stress
  • klebsiella pneumoniae
  • cell therapy
  • stem cells
  • bone marrow
  • anti inflammatory
  • multidrug resistant
  • amino acid
  • staphylococcus aureus