Direct whole-genome sequencing of HIV-1 for clinical drug-resistance analysis and public health surveillance.
Rosa C Coldbeck-ShackleyPenelope J AdamsonDaryn WhybrowCaitlin A SelwayLito E PapanicolasMark TurraLex E X LeongPublished in: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2024)
These findings demonstrate dWGS enhances HIV-1 drug-resistance analysis by quantitative variant detection and improves reconstruction of HIV-1 phylogenies compared to traditional pol sequencing. This work supports that HIV-1 dWGS is a viable option to replace Sanger sequencing for clinical and public health applications.