Integrated Mechanism of Immune Response Modulation by Arctium Lappa L . Fructans Based on Microbiome and Metabolomics Technologies .
Lingyu LiZhichang QiuXinyan BaiWenqing ZhuIftikhar AliChunxia MaZhenjia ZhengXuguang QiaoPublished in: Journal of agricultural and food chemistry (2024)
Arctium lappa L . is widely consumed for its various biological effects, and polysaccharides are its main functional components. The present study aimed to evaluate the immunoregulatory effects of the main polysaccharides from burdock (ALP-1) and reveal the underlying mechanisms. ALP-1 consisted of fructose and glucose (14.57:1) and had a molecular weight of 2757 Da, with typical characteristics of (1 → 2)-linked linear fructans. Oral intake of ALP-1 significantly increased the number of colonic goblet cells, serum immunoglobulin A and immunoglobulin G levels, and fecal secretory immunoglobulin A content as well as up-regulated antioxidant enzymes and increased short chain fatty acid production. In addition, ALP-1 administration regulated pro/anti-inflammatory cytokines (i.e., interleukin (IL)-1β, IL-6, tumor necrosis factor-α, interferon-γ, and IL-10), intestinal microbiota structure, and the spatial information on key metabolites. Some gut-microbiota-mediated metabolic processes were also significantly altered. These results indicated that ALP-1 could exert beneficial effects on immune responses and intestinal health in healthy mice.
Keyphrases
- immune response
- dendritic cells
- fatty acid
- induced apoptosis
- transcription factor
- healthcare
- public health
- anti inflammatory
- mass spectrometry
- health information
- rheumatoid arthritis
- mental health
- toll like receptor
- cell cycle arrest
- ms ms
- single cell
- gene expression
- climate change
- water soluble
- signaling pathway
- metabolic syndrome
- physical activity
- skeletal muscle
- cell death