An enChIP system for the analysis of bacterial genome functions.
Toshitsugu FujitaMiyuki YunoHodaka FujiiPublished in: BMC research notes (2018)
We generated a plasmid inducibly expressing Streptococcus pyogenes dCas9 fused to a 3xFLAG-tag (3xFLAG-dCas9) in bacteria. Inducible expression of 3xFLAG-dCas9 in Escherichia coli was confirmed by immunoblot analysis. We were able to purify specific genomic regions of E. coli preserving their molecular interactions. The system is potentially useful for analysis of interactions between specific genomic regions and their associated molecules in bacterial cells to understand genome functions such as transcription, DNA repair, and DNA recombination.
Keyphrases
- dna repair
- escherichia coli
- dna damage
- biofilm formation
- induced apoptosis
- dna damage response
- copy number
- poor prognosis
- single molecule
- genome wide
- cell cycle arrest
- transcription factor
- crispr cas
- klebsiella pneumoniae
- candida albicans
- circulating tumor
- cell free
- cystic fibrosis
- pseudomonas aeruginosa
- cell death
- cell proliferation
- nucleic acid
- circulating tumor cells