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Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies.

Ana Medel-MartinezAna Redrado-OstaAlejandra Crespo-BarredaMaria Sancho-AlberoLourdes SánchezVictor Sebastian CabezaMaría PardoAntonio de la ViejaPilar Martín-Duque
Published in: Biomolecules (2024)
Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.
Keyphrases
  • mesenchymal stem cells
  • stem cells
  • human milk
  • photodynamic therapy
  • preterm infants
  • fatty acid