Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves.
Tran Duc VietTran Dang XuanTruong Mai VanYusuf AndrianaRamin RayeeHoang-Dung TranPublished in: Medicines (Basel, Switzerland) (2019)
Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50-70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.
Keyphrases
- mass spectrometry
- liquid chromatography
- gas chromatography
- gas chromatography mass spectrometry
- solid phase extraction
- tandem mass spectrometry
- ms ms
- high performance liquid chromatography
- fatty acid
- simultaneous determination
- capillary electrophoresis
- oxidative stress
- high resolution
- liquid chromatography tandem mass spectrometry
- hydrogen peroxide
- ionic liquid
- carbon dioxide
- anti inflammatory
- body composition
- multiple sclerosis
- nitric oxide
- fluorescent probe