Actinidia arguta Extract Containing Myo -Inositol Suppresses TNF- α -Induced VCAM-1 Expression and Monocyte Adhesion to Endothelial Cells via Inhibition of the PTEN/Akt/GSK-3 β and NF- κ B Signaling Pathways.
Jangho LeeJoon ParkKyung-Mo SongYu Geon LeeHyo-Kyoung ChoiPublished in: Journal of medicinal food (2024)
The primary inflammatory process in atherosclerosis, a major contributor to cardiovascular disease, begins with monocyte adhering to vascular endothelial cells. Actinidia arguta (kiwiberry) is an edible fruit that contains various bioactive components. While A. arguta extract (AAE) has been recognized for its anti-inflammatory characteristics, its specific inhibitory effect on early atherogenic events has not been clarified. We used tumor necrosis factor- α (TNF- α )-stimulated human umbilical vein endothelial cells (HUVECs) for an in vitro model. AAE effectively hindered the attachment of THP-1 monocytes and reduced the expression of vascular cell adhesion molecule-1 (VCAM-1) in HUVECs. Transcriptome analysis revealed that AAE treatment upregulated phosphatase and tensin homolog (PTEN), subsequently inhibiting phosphorylation of AKT and glycogen synthase kinase 3 β (GSK3 β ) in HUVECs. AAE further hindered phosphorylation of AKT downstream of the nuclear factor kappa B (NF- κ B) signaling pathway, leading to suppression of target gene expression. Oral administration of AAE suppressed TNF- α -stimulated VCAM-1 expression, monocyte-derived macrophage infiltration, and proinflammatory cytokine expression in C57BL/6 mouse aortas. Myo -inositol, identified as the major compound in AAE, played a key role in suppressing THP-1 monocyte adhesion in HUVECs. These findings suggest that AAE could serve as a nutraceutical for preventing atherosclerosis by inhibiting its initial pathogenesis.
Keyphrases
- signaling pathway
- pi k akt
- endothelial cells
- cell adhesion
- nuclear factor
- poor prognosis
- high glucose
- cardiovascular disease
- epithelial mesenchymal transition
- induced apoptosis
- dendritic cells
- rheumatoid arthritis
- gene expression
- anti inflammatory
- cell proliferation
- binding protein
- peripheral blood
- oxidative stress
- toll like receptor
- vascular endothelial growth factor
- adipose tissue
- type diabetes
- pseudomonas aeruginosa
- inflammatory response
- metabolic syndrome
- single cell
- smoking cessation
- cystic fibrosis
- immune response
- endoplasmic reticulum stress
- combination therapy
- cardiovascular risk factors
- replacement therapy