Insights into the Impact of Gold Nanoclusters Au 10 SG 10 on Human Microglia.
Dusica MaysingerŽeljka Sanader MaršićEvan Rizzel GranAdeola ShoboJun-Ray MacairanIssan ZhangMartina Perić BakulićRodolphe AntoineGerhard MulthaupVlasta Bonačić-KouteckỳPublished in: ACS chemical neuroscience (2022)
The purpose of the current study is to uncover the impact of small liganded gold nanoclusters with 10 gold atoms and 10 glutathione ligands (Au 10 SG 10 ) on several biomarkers in human microglia. We established the links connecting the atomically precise structure of Au 10 SG 10 with their properties and changes in several biomolecules under oxidative stress. Au 10 SG 10 caused the loss of mitochondrial metabolic activity, increased lipid peroxidation and translocation of an alarmin molecule, high mobility group box 1 (HMGB1), from the nucleus to the cytosol. Molecular modeling provided an insight into the location of amino acid interaction sites with Au 10 SG 10 and the nature of bonds participating in these interactions. We show that Au 10 SG 10 can bind directly to the defined sites of reduced, oxidized, and acetylated HMGB1. Further studies with similar complementary approaches merging live-cell analyses, determination of biomarkers, and cell functions could lead to optimized gold nanoclusters best suited for diagnostic and bioimaging purposes in neuroscience.
Keyphrases
- sensitive detection
- quantum dots
- oxidative stress
- reduced graphene oxide
- endothelial cells
- inflammatory response
- silver nanoparticles
- transcription factor
- induced pluripotent stem cells
- neuropathic pain
- dna damage
- cell therapy
- stem cells
- gold nanoparticles
- mass spectrometry
- pluripotent stem cells
- signaling pathway
- spinal cord
- label free
- binding protein
- high resolution
- single molecule