Bone marrow aspirate concentrate quality is affected by age and harvest site.
Carola CavalloAngelo BoffaLaura de GirolamoGiulia MerliElizaveta KonLuca CattiniEmma SantoBrunella GrigoloGiuseppe FilardoPublished in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA (2022)
Harvest site and age can affect the quality of BMAC. BMSCs obtained from iliac crest and proximal tibia present comparable mesenchymal markers expression as well as osteogenic and chondrogenic differentiation potential, but iliac crest BMAC presents a four times higher number of mononucleated cells with significantly higher clonogenic capacity compared to the tibia. BMAC of younger patients also had a three-time higher number of mononucleated cells. The identification of BMAC characteristics could help to optimize its preparation and to identify the most suitable indications for this orthobiologic treatment in the clinical practice.
Keyphrases
- bone marrow
- induced apoptosis
- mesenchymal stem cells
- cell cycle arrest
- clinical practice
- end stage renal disease
- newly diagnosed
- chronic kidney disease
- poor prognosis
- stem cells
- prognostic factors
- endoplasmic reticulum stress
- cell death
- pi k akt
- cell proliferation
- mass spectrometry
- climate change
- high resolution
- replacement therapy
- liquid chromatography
- combination therapy
- binding protein
- bioinformatics analysis