Unraveling the Metabolic Alterations Induced by Zika Infection in Prostate Epithelial (PNT1a) and Adenocarcinoma (PC-3) Cell Lines.
Jeany DelafioriAlessandra V de S FariaArthur N de OliveiraGeovana M SalesFlávia Luísa Dias-AudibertRodrigo Ramos CatharinoPublished in: Journal of proteome research (2022)
The outbreak of Zika virus infection in 2016 led to the identification of its presence in several types of biofluids, including semen. Later discoveries associated Zika infection with sexual transmission and persistent replication in cells of the male reproductive tract. Prostate epithelial and carcinoma cells are favorable to virus replication, with studies pointing to transcriptomics alterations of immune and inflammation genes upon persistence. However, metabolome alterations promoted by the Zika virus in prostate cells are unknown. Given its chronic effects and oncolytic potential, we aim to investigate the metabolic alterations induced by the Zika virus in prostate epithelial (PNT1a) and adenocarcinoma (PC-3) cells using an untargeted metabolomics approach and high-resolution mass spectrometry. PNT1a cells were viable up to 15 days post ZIKV infection, in contrast to its antiproliferative effect in the PC-3 cell lineage. Remarkable alterations in the PNT1a cell metabolism were observed upon infection, especially regarding glycerolipids, fatty acids, and acylcarnitines, which could be related to viral cellular resource exploitation, in addition to the over-time increase in oxidative stress metabolites associated with carcinogenesis. The upregulation of FA20:5 at 5 dpi in PC-3 cells corroborates the antiproliferative effect observed since this metabolite was previously reported to induce PC-3 cell death. Overall, Zika virus promotes extensive lipid alterations on both PNT1a and PC-3 cells, promoting different outcomes based on the cellular metabolic state.
Keyphrases
- zika virus
- induced apoptosis
- dengue virus
- prostate cancer
- cell cycle arrest
- oxidative stress
- aedes aegypti
- cell death
- single cell
- high resolution mass spectrometry
- benign prostatic hyperplasia
- squamous cell carcinoma
- endoplasmic reticulum stress
- signaling pathway
- fatty acid
- sars cov
- stem cells
- liquid chromatography
- mental health
- gene expression
- pi k akt
- type diabetes
- climate change
- radiation therapy
- dna damage
- dna methylation
- mesenchymal stem cells
- diabetic rats
- human health
- gas chromatography mass spectrometry