A face-to-face comparison of claudin-5 transduced human brain endothelial (hCMEC/D3) cells with porcine brain endothelial cells as blood-brain barrier models for drug transport studies.

Birthe GerickeKerstin RömermannAndreas NoackSandra NoackJessica KronenbergIngolf Ernst BlasigWolfgang Löscher

Published in: Fluids and barriers of the CNS (2020)

The claudin-5 transduced hCMEC/D3 cells provide a tool to studying the contribution of claudin-5 to barrier tightness and how this can be further enhanced by additional transfections or other manipulations of this widely used in vitro model of the BBB.

Keyphrases

blood brain barrier
induced apoptosis
endothelial cells
cell cycle arrest
cerebral ischemia
emergency department
cell death
endoplasmic reticulum stress
high glucose
functional connectivity
adverse drug