Magnetically responsive nanoplatform targeting circRNA circ_0058051 inhibits hepatocellular carcinoma progression.
Song YouZijin LuoNiangmei ChengMing WuYongping LaiFei WangXiaoyuan ZhengYingchao WangXiaolong LiuJingfeng LiuBixing ZhaoPublished in: Drug delivery and translational research (2022)
Circular RNAs (circRNAs) are a class of highly stable and closed-loop noncoding RNA that are involved in the occurrence and development of hepatocellular carcinoma (HCC). However, little is known about the therapeutic role of circRNAs in HCC. We found that high circ_0058051 expression was negatively correlated with the prognosis of HCC patients. Circ_0058051 knockdown attenuated the proliferation and colony formation, meanwhile inhibited migration of HCC cells. Circ_0058051 may be used as a target for HCC gene therapy. We synthesized a novel small interfering RNA (siRNA) delivery system, PEG-PCL-PEI-C14-SPIONs (PPPCSs), based on superparamagnetic iron oxide nanoparticles (SPIONs). PPPCSs protected the siRNA of circ_0058051 from degradation in serum and effectively delivered siRNA into SMMC-7721 cells. Meanwhile, intravenous injection of the PPPCSs/siRNA complex could inhibit tumor growth in the subcutaneous tumor model. In addition, the nanocomposite is not toxic to the organs of nude mice. The above results show that PPPCSs/si-circ_0058051 complex may provide a novel and promising method of HCC treatment.
Keyphrases
- cancer therapy
- iron oxide nanoparticles
- induced apoptosis
- drug delivery
- gene therapy
- cell cycle arrest
- end stage renal disease
- newly diagnosed
- ejection fraction
- risk assessment
- chronic kidney disease
- poor prognosis
- signaling pathway
- endoplasmic reticulum stress
- oxidative stress
- gold nanoparticles
- high dose
- type diabetes
- low dose
- cell death
- peritoneal dialysis
- cell proliferation
- binding protein
- patient reported outcomes
- room temperature
- smoking cessation
- nucleic acid
- ultrasound guided