Engineering for an HPV 9-valent vaccine candidate using genomic constitutive over-expression and low lipopolysaccharide levels in Escherichia coli cells.
Kaihang WangLizhi ZhouTingting ChenQiong LiJiajia LiLiqin LiuYuqian LiJie SunTingting LiYingbin WangZhibo KongQingbing ZhengJun ZhangHai YuYing GuNingshao XiaShao-Wei LiPublished in: Microbial cell factories (2021)
Reengineering the LPS synthesis loci in the E. coli ER2566 strain through chromosomal integration of expression cassettes has potential uses for the production of a 9-valent HPV vaccine candidate, with markedly reduced residual endotoxin levels. Our results offer a new strategy for recombinant E. coli strain construction, engineering, and the development of suitable recombinant protein drugs.
Keyphrases
- escherichia coli
- poor prognosis
- inflammatory response
- binding protein
- induced apoptosis
- high grade
- copy number
- cell cycle arrest
- long non coding rna
- toll like receptor
- genome wide
- amino acid
- signaling pathway
- lps induced
- biofilm formation
- protein protein
- immune response
- estrogen receptor
- cell death
- anti inflammatory
- risk assessment
- cervical cancer screening
- cell proliferation
- multidrug resistant
- drug induced
- genome wide association