Black Sorghum Phenolic Extract Regulates Expression of Genes Associated with Oxidative Stress and Inflammation in Human Endothelial Cells.
Nidhish FrancisShiwangini RaoChristopher BlanchardAbishek Bommannan SanthakumarPublished in: Molecules (Basel, Switzerland) (2019)
Oxidative stress is one of the primary factors leading to endothelial dysfunction, a major underlying cause of vascular disorders. This study aims to understand the key signalling pathways regulated by sorghum (Shawaya short black 1 variety; characterised to be very high in its antioxidant activity) under oxidative stress in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were pre-treated with non-cytotoxic concentrations of phenolic-rich black sorghum extract (BSE) prior to induction of oxidative stress using hydrogen peroxide (H2O2). Treatment with BSE upregulated the expression of heme oxygenase 1 (HO1) and endothelial nitric oxide synthase (eNOS) and downregulated the levels of NADPH oxidase 4 (NOX4). BSE treatment significantly reduced the expression of pro-inflammatory mediators such as monocyte chemoattractant protein 1 (MCP1) and intracellular adhesion molecule 1 (ICAM1). Results from this study suggest that phenolic-rich BSE may reduce oxidative stress by regulating pro- and antioxidant signalling pathways and the expression of inflammatory mediators linked to endothelial dysfunction under oxidative stress.
Keyphrases
- oxidative stress
- endothelial cells
- poor prognosis
- diabetic rats
- ischemia reperfusion injury
- dna damage
- induced apoptosis
- nitric oxide synthase
- high glucose
- nitric oxide
- binding protein
- heat shock
- long non coding rna
- dendritic cells
- pseudomonas aeruginosa
- cystic fibrosis
- cell proliferation
- escherichia coli
- reactive oxygen species
- peripheral blood
- staphylococcus aureus
- candida albicans
- newly diagnosed
- pi k akt