Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori.
Sandy R PernitzschMona AlzheimerBelinda U BremerMarie Robbe-SauleHilde De ReuseCynthia Mira SharmaPublished in: Nature communications (2021)
The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase variation by also modulating the expression of a gene (hp0102) that is co-transcribed with tlpB. The hp0102 gene encodes a glycosyltransferase required for LPS O-chain biosynthesis and in vivo colonization of the mouse stomach. The G-repeat length defines a gradual (rather than ON/OFF) control of LPS biosynthesis by RepG, and leads to gradual resistance to a membrane-targeting antibiotic. Thus, RepG-mediated modulation of LPS structure might impact host immune recognition and antibiotic sensitivity, thereby helping H. pylori to adapt and persist in the host.
Keyphrases
- helicobacter pylori
- inflammatory response
- anti inflammatory
- poor prognosis
- helicobacter pylori infection
- lps induced
- toll like receptor
- binding protein
- copy number
- cell wall
- cancer therapy
- genome wide
- transcription factor
- genome wide identification
- signaling pathway
- long non coding rna
- gene expression
- dna methylation
- genome wide analysis