High Yield of RNA Sequencing for Targetable Kinase Fusions in Lung Adenocarcinomas with No Mitogenic Driver Alteration Detected by DNA Sequencing and Low Tumor Mutation Burden.

Ryma Benayed Michael D OffinKerry MullaneyPurvil Sukhadia Kelly RiosPatrice DesmeulesRyan N PtashkinHelen WonJason ChangDarragh HalpennyAlison M Schram Charles M RudinDavid M HymanMaria E ArcilaMichael F BergerAhmet ZehirMark G KrisAlexander E DrilonMarc Ladanyi

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2019)

Targeted RNAseq assays should be used in all cases that appear driver negative by DNAseq assays to ensure comprehensive detection of actionable gene rearrangements. Furthermore, we observed a significant enrichment for fusions in DNAseq driver-negative samples with low TMB, supporting the prioritization of such cases for additional RNAseq.See related commentary by Davies and Aisner, p. 4586.

Keyphrases

single cell
high throughput
copy number
genome wide
cancer therapy
single molecule
gene expression
protein kinase
transcription factor
nucleic acid