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In vivo genome editing using the Cpf1 ortholog derived from Eubacterium eligens.

Woo-Chan AhnKwang-Hyun ParkIn Seon BakHyung-Nam SongYan AnSu-Jin LeeMira JungKyeong-Won YooDae-Yeul YuYong-Sam KimByung-Ha OhEui-Jeon Woo
Published in: Scientific reports (2019)
Cpf1 is an RNA-guided endonuclease that can be programmed to cleave DNA targets. Specific features, such as containing a short crRNA, creating a staggered cleavage pattern and having a low off-target rate, render Cpf1 a promising gene-editing tool. Here, we present a new Cpf1 ortholog, EeCpf1, as a genome-editing tool; this ortholog is derived from the gut bacterial species Eubacterium eligens. EeCpf1 exhibits a higher cleavage activity with the Mn2+ metal cofactor and efficiently cuts the target DNA with an engineered, nucleotide extended crRNA at the 5' target site. When mouse blastocysts were injected with multitargeting crRNAs against the IL2R-γ gene, an essential gene for immunodeficient mouse model production, EeCpf1 efficiently generated IL2R-γ knockout mice. For the first time, these results demonstrate that EeCpf1 can be used as an in vivo gene-editing tool for the production of knockout mice. The utilization of engineered crRNA with multiple target sites will help to explore the in vivo DNA cleavage activities of Cpf1 orthologs from other species that have not been demonstrated.
Keyphrases
  • genome editing
  • crispr cas
  • circulating tumor
  • cell free
  • mouse model
  • single molecule
  • dna binding
  • genome wide
  • copy number
  • gene expression
  • dna damage
  • genome wide identification
  • genetic diversity
  • genome wide analysis