In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells.
Mehmet Enes ArslanArzu TatarÖzge Çağlar Yıldırımİrfan Oğuz ŞahinOzlem Ozdemir TozluErdal SonmezAhmet HacımuftuogluMetin AcikyildizFatime GeyikoğluAdil MardingluHasan TürkezPublished in: Materials (Basel, Switzerland) (2022)
Nanobiotechnology influences many different areas, including the medical, food, energy, clothing, and cosmetics industries. Considering the wide usage of nanomaterials, it is necessary to investigate the toxicity potentials of specific nanosized molecules. Boron-containing nanoparticles (NPs) are attracting much interest from scientists due to their unique physicochemical properties. However, there is limited information concerning the toxicity of boron-containing NPs, including cobalt boride (Co 2 B) NPs. Therefore, in this study, Co 2 B NPs were characterized using X-ray crystallography (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. Then, we performed 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) release, and neutral red (NR) assays for assessing cell viability against Co 2 B NP exposure on cultured human pulmonary alveolar epithelial cells (HPAEpiC). In addition, whole-genome microarray analysis was carried out to reveal the global gene expression differentiation of HPAEpiC cells after Co 2 B NP application. The cell viability tests unveiled an IC50 value for Co 2 B NPs of 310.353 mg/L. The results of our microarray analysis displayed 719 gene expression differentiations (FC ≥ 2) among the analyzed 40,000 genes. The performed visualization and integrated discovery (DAVID) analysis revealed that there were interactions between various gene pathways and administration of the NPs. Based on gene ontology biological processes analysis, we found that the P53 signaling pathway, cell cycle, and cancer-affecting genes were mostly affected by the Co 2 B NPs. In conclusion, we suggested that Co 2 B NPs would be a safe and effective nanomolecule for industrial applications, particularly for medical purposes.
Keyphrases
- gene expression
- cell cycle
- genome wide
- oxide nanoparticles
- induced apoptosis
- endothelial cells
- high resolution
- dna methylation
- healthcare
- pulmonary hypertension
- squamous cell carcinoma
- oxidative stress
- electron microscopy
- risk assessment
- high throughput
- single cell
- wastewater treatment
- endoplasmic reticulum stress
- cell death
- transcription factor
- dual energy
- ionic liquid
- social media
- pluripotent stem cells