Microcella aerolata sp. nov., isolated from electronic waste-associated bioaerosols.

A Gram-positive, non-motile, non-spore-forming and short rod-shaped actinomycete strain, designated GA224 T , was isolated from electronic waste-associated bioaerosols. The optimal growth conditions for this isolate, a facultatively anaerobic bacterium, were 37 °C and pH 8.0. The cell-wall peptidoglycan type was B2γ, with 2,4-diaminobutyric acid (DAB) as the diamino acids, while the major menaquinone was MK-12. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and an unidentified lipid. The major cellular fatty acids were anteiso-C 15:0 and iso-C 16:0 . Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GA224 T fell within the genus Microcella. The draft genome of strain GA224 T comprised 2,495,189 bp with a G + C content of 72.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain GA224 T and the type strain of the type species of Microcella species were lower than 95% and 70%, respectively. Based on the phenotypic, chemotaxonomic and genomic data, strain GA224 T represents a novel species, for which the name Microcella aerolata sp. nov. is proposed, with GA224 T as the type strain (= GDMCC 1.2165  T  = JCM 34462  T ).