A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway.

Jennifer T NguyenKennedy K RiebschlegerKatelyn V BrownNina M GorgijevskaStephen Eric Nybo

Published in: Biotechnology journal (2021)

Altogether, this system is compatible with the BioBricks [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis.

Keyphrases

poor prognosis
genome wide
copy number
high throughput
molecular docking
gene expression
long non coding rna
dna methylation
binding protein
cell wall