Comparison between Listeria sensu stricto and Listeria sensu lato strains identifies novel determinants involved in infection.
Jakob SchardtGrant JonesStefanie Müller-HerbstKristina SchauerSarah E F D'OrazioThilo M FuchsPublished in: Scientific reports (2017)
The human pathogen L. monocytogenes and the animal pathogen L. ivanovii, together with four other species isolated from symptom-free animals, form the "Listeria sensu stricto" clade. The members of the second clade, "Listeria sensu lato", are believed to be solely environmental bacteria without the ability to colonize mammalian hosts. To identify novel determinants that contribute to infection by L. monocytogenes, the causative agent of the foodborne disease listeriosis, we performed a genome comparison of the two clades and found 151 candidate genes that are conserved in the Listeria sensu stricto species. Two factors were investigated further in vitro and in vivo. A mutant lacking an ATP-binding cassette transporter exhibited defective adhesion and invasion of human Caco-2 cells. Using a mouse model of foodborne L. monocytogenes infection, a reduced number of the mutant strain compared to the parental strain was observed in the small intestine and the liver. Another mutant with a defective 1,2-propanediol degradation pathway showed reduced persistence in the stool of infected mice, suggesting a role of 1,2-propanediol as a carbon and energy source of listeriae during infection. These findings reveal the relevance of novel factors for the colonization process of L. monocytogenes.
Keyphrases
- listeria monocytogenes
- endothelial cells
- mouse model
- genome wide
- wild type
- induced apoptosis
- escherichia coli
- oxidative stress
- induced pluripotent stem cells
- gene expression
- cell cycle arrest
- pluripotent stem cells
- cell migration
- candida albicans
- staphylococcus aureus
- cell death
- cystic fibrosis
- dna methylation
- mass spectrometry
- pseudomonas aeruginosa
- atomic force microscopy
- high speed