CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries.
Florian HeigwerTianzuo ZhanMarco BreinigJan WinterDirk BrügemannSvenja LeibleMichael BoutrosPublished in: Genome biology (2016)
CLD predicts a high fraction of functional sgRNAs and is publicly available at https://github.com/boutroslab/cld.