In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue.
Stella Maria Andrade Gomes BarretoCesar Orlando Muñoz CadavidRafael Amir de Oliveira MouraGiovanna Melo Martins SilvaSamara Vitória Ferreira de AraújoJean Antônio Aderaldo da Silva FilhoHugo Alexandre DE Oliveira RochaRiva de Paula OliveiraRaquel Brandt GiordaniMárcio FerrariPublished in: Biomolecules (2020)
Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.
Keyphrases
- oxidative stress
- wastewater treatment
- heavy metals
- reactive oxygen species
- dna damage
- diabetic rats
- ischemia reperfusion injury
- induced apoptosis
- amino acid
- anti inflammatory
- poor prognosis
- risk assessment
- cell proliferation
- human health
- signaling pathway
- heat shock
- metal organic framework
- climate change
- heat shock protein