Transcriptomic signature of painful human neurofibromatosis type 2 schwannomas.
Phanidhar KukutlaSherif G AhmedDaniel M DuBreuilAhmed AbdelnabiMurat CetinbasGiulia FulciBerent AldikactiAnat Stemmer-RachamimovScott R PlotkinBrian WaingerRuslan I SadreyevGary J BrennerPublished in: Annals of clinical and translational neurology (2021)
Schwannomas are benign neoplasms that can cause gain- and loss-of-function neurological phenotypes, including severe, intractable pain. To investigate the molecular mechanisms underlying schwannoma-associated pain we compared the RNA sequencing profile of painful and non-painful schwannomas from NF2 patients. Distinct segregation of painful and non-painful tumors by gene expression patterns was observed. Differential expression analysis showed the upregulation of fibroblast growth factor 7 (FGF7) in painful schwannomas. Behavioral support for this finding was observed using a xenograft human NF2-schwannoma model in nude mice. In this model, over-expression of FGF7 in intra-sciatically implanted NF2 tumor cells generated pain behavior compared with controls.
Keyphrases
- signaling pathway
- chronic pain
- gene expression
- endothelial cells
- pain management
- lps induced
- poor prognosis
- neuropathic pain
- oxidative stress
- end stage renal disease
- pi k akt
- single cell
- newly diagnosed
- cell proliferation
- nuclear factor
- dna methylation
- chronic kidney disease
- metabolic syndrome
- type diabetes
- early onset
- skeletal muscle
- subarachnoid hemorrhage
- immune response
- insulin resistance
- brain injury
- high fat diet induced
- blood brain barrier
- toll like receptor