Effects of Culture Mechanism of Cinnamomum kanehirae and C. camphora on the Expression of Genes Related to Terpene Biosynthesis in Antrodia cinnamomea .
Zhang ZhangYi WangXiao-Long YuanYa-Na LuoMa-Niya LuoYuan ZhengPublished in: Mycobiology (2022)
The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome. The results were assembled de novo , and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase ( ACS2 and ACS4 ), hydroxymethylglutaryl CoA reductase ( HMGR ), farnesyl transferase ( FTase ), and squalene synthase ( SQS ) were found to be upregulated in XZJ (twig of C. camphora ) and NZJ (twig of C. kanehirae ). Moreover, ACS5 and 2,3-oxidized squalene cyclase ( OCS ) were highly expressed in NZJ, while heme IX farnesyl transferase ( IX-FIT ) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS , and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.
Keyphrases
- acute coronary syndrome
- genome wide
- genome wide identification
- transcription factor
- healthcare
- big data
- mental health
- gene expression
- bioinformatics analysis
- dna methylation
- emergency department
- electronic health record
- fatty acid
- risk assessment
- genome wide analysis
- single cell
- copy number
- machine learning
- artificial intelligence
- human health
- drug discovery
- amino acid