Differential Expression of DUB Genes in Ovarian Cells Treated with Di-2-Ethylhexyl Phthalate.
Da-Hye LeeJun-Hyeok ParkJihye ChoiKyung-Ju LeeBo-Seong YunKwang-Hyun BaekPublished in: International journal of molecular sciences (2020)
Premature ovarian failure (POF) is defined as loss of ovarian function in women less than 40 years of age. The causes of POF are diverse and include environmental factors. Di-2-ethylhexyl phthalate (DEHP) is one factor that may cause POF. The ubiquitin-proteasome system maintains intracellular balance by promoting or inhibiting protein degradation. To investigate the differential expressions of deubiquitinating enzyme (DUB) genes in patients with POF, we developed two in vitro POF models by treating A2780 or OVCAR5 with DEHP. Using these models, a multiplex RT-PCR system for DUB genes was applied to identify biomarkers by comparing expression patterns and DUB mRNA levels; multiplex RT-PCR results were validated by qRT-PCR and Western blotting analyses. Observed differential expression levels of several DUB genes including USP12, COPS5, ATXN3L, USP49, and USP34 in A2780 and OVCAR5 cells at the mRNA and protein levels suggest that they should be investigated as potential biomarkers of POF.
Keyphrases
- induced apoptosis
- genome wide
- real time pcr
- binding protein
- genome wide identification
- bioinformatics analysis
- cell cycle arrest
- high throughput
- genome wide analysis
- poor prognosis
- cell death
- polycystic ovary syndrome
- transcription factor
- small molecule
- endoplasmic reticulum stress
- pregnant women
- escherichia coli
- staphylococcus aureus
- adipose tissue
- high resolution
- amino acid