bta-miR-2904 inhibits bovine viral diarrhea virus replication by targeting viral-infection-induced autophagy via ATG13.
Ningning YangNana HuJiangwei ZhangJihai YiZhen WangYong WangPeng WuNingning YangPublished in: Archives of virology (2022)
MicroRNAs (miRNAs) are endogenous small and noncoding RNA molecules (18-25 nt) that can regulate expression of their target genes post-transcriptionally. Previously, using high-throughput sequencing data obtained on a Solexa platform, we found that Bos taurus bta-miR-2904 (miR-2904) was significantly upregulated in Madin-Darby bovine kidney (MDBK) cells infected with bovine viral diarrhea virus (BVDV) strain NADL at 2, 6, and 18 h postinfection (hpi) compared to uninfected MDBK cells. Moreover, miR-2904 overexpression significantly reduced BVDV replication. However, the mechanism by which miR-2904 inhibits viral replication remains unclear. In this study, we used electron microscopy, laser confocal microscopy, dual-luciferase reporter analysis, real-time PCR, and Western blot assays to investigate the effect of the miR-2904 expression on BVDV NADL replication and virus-infection-induced autophagy. The results indicate that miR-2904 inhibits autophagy of MDBK cells by targeting autophagy-related gene 13 (ATG13), and overexpression of miR-2904 inhibited the replication of BVDV NADL.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- poor prognosis
- cell death
- induced apoptosis
- endoplasmic reticulum stress
- oxidative stress
- sars cov
- cell cycle arrest
- high throughput
- genome wide
- diabetic rats
- transcription factor
- gene expression
- high resolution
- machine learning
- dna methylation
- pi k akt
- mass spectrometry
- south africa
- real time pcr
- deep learning
- data analysis
- single cell
- drug induced
- endothelial cells
- copy number