Erroneous expression of NKG2D on granulocytes detected by phycoerythrin-conjugated clone 149810 antibody.
Guranda ChitadzeMarcus LettauChristian PetersStefanie LueckeCharlotte FlühElgar Susanne QuabiusMichael SynowitzJanka Held-FeindtDieter KabelitzPublished in: Cytometry. Part B, Clinical cytometry (2021)
Our results uncover an erroneous and false positive reactivity of PE-labeled (but not of APC-labeled or unconjugated) anti-NKG2D antibody 149810 on granulocytes from dexamethasone-treated GBM patients and raise a note of caution for studies of NKG2D expression on non-lymphoid cells.
Keyphrases
- poor prognosis
- end stage renal disease
- newly diagnosed
- nk cells
- natural killer cells
- ejection fraction
- induced apoptosis
- chronic kidney disease
- low dose
- peritoneal dialysis
- binding protein
- prognostic factors
- cell cycle arrest
- high dose
- patient reported outcomes
- photodynamic therapy
- oxidative stress
- cell death
- cell proliferation
- positron emission tomography
- pi k akt
- pet ct