Transcriptomic and Metabolomic Analyses Providing Insights into the Coloring Mechanism of Docynia delavayi .
Yuchang WangYuyang SongDawei WangPublished in: Foods (Basel, Switzerland) (2022)
The metabolome and transcriptome profiles of three different variations of mature Docynia delavayi fruit were synthesized to reveal their fruit color formation mechanism. A total of 787 secondary metabolites containing 149 flavonoid metabolites, most of which were flavonoids and flavonols, were identified in the three variations using ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS), and we found that the secondary metabolites cyanidin-3-O-galactoside and cyanidin-3-O-glucoside were the major coloring substances in D. delavayi . This was associated with the significant upregulation of the structural genes F3H and F3'H in the anthocyanin synthesis pathway and the control genes WRKY, MYB, bZIP, bHLH, and NAC in RP. F3'H expression may play a significant role in the selection of components for anthocyanin synthesis. Our results contribute to breeding and nutritional research in D. delavayi and provide insight into metabolite studies of the anthocyanin biosynthetic pathway.
Keyphrases
- ms ms
- transcription factor
- liquid chromatography tandem mass spectrometry
- genome wide identification
- genome wide
- poor prognosis
- single cell
- simultaneous determination
- genome wide analysis
- rna seq
- dna methylation
- bioinformatics analysis
- gene expression
- high resolution
- drinking water
- cell proliferation
- long non coding rna
- binding protein
- solid phase extraction
- liquid chromatography