Vascular Tissue Specific miRNA Profiles Reveal Novel Correlations with Risk Factors in Coronary Artery Disease.
Katrina Daila NeiburgaBaiba VilneSabine BauerDario BongiovanniTilman ZieglerMark LachmannSimon WengertJohann S HaweUlrich GüldenerAnnie M WesterlundLing LiShichao PangChuhua YangKathrin SaarNorbert HuebnerLars Maegdefesselnull DigiMed Bayern ConsortiumRüdiger LangeMarkus KraneHeribert SchunkertMoritz von ScheidtPublished in: Biomolecules (2021)
Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide. Non-coding RNAs have already been linked to CVD development and progression. While microRNAs (miRs) have been well studied in blood samples, there is little data on tissue-specific miRs in cardiovascular relevant tissues and their relation to cardiovascular risk factors. Tissue-specific miRs derived from Arteria mammaria interna (IMA) from 192 coronary artery disease (CAD) patients undergoing coronary artery bypass grafting (CABG) were analyzed. The aims of the study were 1) to establish a reference atlas which can be utilized for identification of novel diagnostic biomarkers and potential therapeutic targets, and 2) to relate these miRs to cardiovascular risk factors. Overall, 393 individual miRs showed sufficient expression levels and passed quality control for further analysis. We identified 17 miRs-miR-10b-3p, miR-10-5p, miR-17-3p, miR-21-5p, miR-151a-5p, miR-181a-5p, miR-185-5p, miR-194-5p, miR-199a-3p, miR-199b-3p, miR-212-3p, miR-363-3p, miR-548d-5p, miR-744-5p, miR-3117-3p, miR-5683 and miR-5701-significantly correlated with cardiovascular risk factors (correlation coefficient >0.2 in both directions, p-value (p < 0.006, false discovery rate (FDR) <0.05). Of particular interest, miR-5701 was positively correlated with hypertension, hypercholesterolemia, and diabetes. In addition, we found that miR-629-5p and miR-98-5p were significantly correlated with acute myocardial infarction. We provide a first atlas of miR profiles in IMA samples from CAD patients. In perspective, these miRs might play an important role in improved risk assessment, mechanistic disease understanding and local therapy of CAD.
Keyphrases
- cardiovascular risk factors
- coronary artery disease
- coronary artery bypass grafting
- cardiovascular disease
- long non coding rna
- cell proliferation
- percutaneous coronary intervention
- long noncoding rna
- cardiovascular events
- metabolic syndrome
- acute myocardial infarction
- poor prognosis
- risk assessment
- risk factors
- end stage renal disease
- patients undergoing
- quality control
- single cell
- blood pressure
- type diabetes
- chronic kidney disease
- stem cells
- gene expression
- machine learning
- acute coronary syndrome
- peritoneal dialysis
- human health
- insulin resistance
- coronary artery bypass
- genome wide
- adipose tissue
- heavy metals
- bone marrow
- high throughput
- left ventricular
- patient reported
- magnetic resonance imaging
- solid state
- smoking cessation
- cell therapy
- deep learning
- climate change