CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.
Yuehong ShenYuling ChenYuntao LinYicun LiPengfei LiuBiru ZhangYufan WangKing-Chi ChanNai-Ki MakMichael KahnRobert Zhong QiHongyu YangPublished in: Cell death & disease (2023)
In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.
Keyphrases
- cell cycle
- poor prognosis
- lymph node metastasis
- cell proliferation
- papillary thyroid
- signaling pathway
- induced apoptosis
- squamous cell carcinoma
- binding protein
- single cell
- genome wide
- transcription factor
- pi k akt
- gene expression
- cell cycle arrest
- long non coding rna
- copy number
- bone marrow
- climate change
- cell therapy
- cell death
- endoplasmic reticulum stress
- dna methylation