Controllable Assembly of a Quantum Dot-Based Aptasensor Guided by CRISPR/Cas12a for Direct Measurement of Circulating Tumor Cells in Human Blood.
Qian ZhangXin GaoYi-Ping HoMeng LiuYun HanDong-Ling LiHui-Min YuanShuangshuang ZhangPublished in: Nano letters (2024)
Accurate and sensitive analysis of circulating tumor cells (CTCs) in human blood provides a non-invasive approach for the evaluation of cancer metastasis and early cancer diagnosis. Herein, we demonstrate the controllable assembly of a quantum dot (QD)-based aptasensor guided by CRISPR/Cas12a for direct measurement of CTCs in human blood. We introduce a magnetic bead@activator/recognizer duplex core-shell structure to construct a multifunctional platform for the capture and direct detection of CTCs in human blood, without the need for additional CTC release and re-identification steps. Notably, the introduction of magnetic separation ensures that only a target-induced free activator can initiate the downstream catalysis, efficiently avoiding the undesired catalysis triggered by inappropriate recognition of the activator/recognizer duplex structure by crRNAs. This aptasensor achieves high CTC-capture efficiency (82.72%) and sensitive detection of CTCs with a limit of detection of 2 cells mL -1 in human blood, holding great promise for the liquid biopsy of cancers.
Keyphrases
- circulating tumor cells
- endothelial cells
- sensitive detection
- crispr cas
- induced pluripotent stem cells
- circulating tumor
- quantum dots
- genome editing
- papillary thyroid
- squamous cell carcinoma
- loop mediated isothermal amplification
- immune response
- label free
- drug delivery
- inflammatory response
- high throughput
- mass spectrometry
- artificial intelligence
- drug induced
- lymph node metastasis
- high resolution
- single cell
- metal organic framework