Comparing Methods to Genetically Engineer Bacteriophage and Increase Host Range.
Christopher J KovacsAlessia AntonacciAbigail GrahamFaye JessupWilliam RankinBrianna BraskoFiona MaguireMichael A WashingtonSiang C KuaF John BurpoJason C BarnhillPublished in: Military medicine (2024)
Each of the phage engineering techniques addressed herein varies in amount of effort and overall success rate. CRISPR-Cas-facilitated modification of phage genomes presents a highly efficient method that does not require a lengthy purification and screening process. It therefore appears to be the method best suited for military medical applications.