Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells.
Kristel SeppMartin LeeMarie T J BluntzerG Vignir HelgasonAlison N HulmeValerie G BruntonPublished in: Journal of medicinal chemistry (2019)
Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.
Keyphrases
- label free
- chronic myeloid leukemia
- high resolution
- induced apoptosis
- endothelial cells
- cell cycle arrest
- mass spectrometry
- social media
- deep learning
- induced pluripotent stem cells
- reactive oxygen species
- signaling pathway
- pluripotent stem cells
- solid phase extraction
- high speed
- monte carlo
- molecularly imprinted
- photodynamic therapy
- optical coherence tomography
- pi k akt
- liquid chromatography